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Oxidative Stress Products

octobre 17 | news, Oxidative Stress, Products | admin

Oxidative stress represents an imbalance between reactive oxygen species (ROS) and cell system’s ability to detoxify the reactive intermediates or to repair the resulting damage. Disturbances in the normal state can cause toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids, and DNA.

Nowadays, oxidative stress may involved in cell perturbation leading to various diseases like atherosclerosis, heart failure, myocardial infarction, Alzheimer’s disease, schizophrenia…

Advanced BioDesign offer you a wide range of product in order to monitor oxidative stress in your cells or tissues following specific marker:

Download our product flyer: Oxidative Stress Product.

Advanced BioDesign Team

Tagged Advanced BioDesign, Oxidative Stress, Products

HISbind Plate Kit

juin 27 | news, Products, Protein Research | admin

Advanced BioDesign HISbind Plate Kit provides a platform for capture, enzymatic assay and purification of recombinant histidine tagged protein.

Tagged 6-His, ELISA, histidine, nickel, plate

EIA HRP Substrate Kit

mai 20 | news, Products, Protein Research | admin

Horseradish Peroxydase (HRP) is a 40kDa protein that catalyzes the oxidation of hydrogen peroxide (H2O2) that, in the presence of substrate, results in a colored or fluorescent product. HRP is one of the most commonly used protein-markers in protein research and TMB (3, 3’, 4, 4’ – tetramethylbenzidine) is the most common substrate used in ELISA. Reaction between the acceptor and oxidized H2O2 will produce a blue colored solution. Once the desired intensity is obtained, the reaction is stopped with an acidic solution (STOP Solution). The reaction’s intensity can be monitored with a 96 well plate reader at 450nm.

Tagged EIA, ELISA, HRP Substrate, TMB

ECL Substrate Kit

mai 20 | news, Products, Protein Research | admin

Advanced BioDesign ECL Western Blotting Substrate is using a new luminol enhancer for boosting signals without increasing the background.

Tagged chemiluminescent, ECL, HRP Substrate, Western Blot

Hexanoyl-lysine (HEL) monoclonal antibody

mars 23 | Oxidative Stress, Products | admin

Hexanoyl-lysine: A novel biomarker for early stage lipid peroxydation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HpODE) is found to be covalently bound to proteins. Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

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02-0702: Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) (20µg)

Tagged HEL, Hexanoyllysine, Oxidative Stress, protein oxidation

Hexanoyl-Lysine Adduct(HEL) ELISA kit

mars 14 | Oxidative Stress, Products | admin

Hexanoyl-Lysine Adduct: A early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

Hexanoyllsyine formation process

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02-0701: Hexanoyl-Lys(HEL) ELISA kit

Tagged HEL, Hexanoyllysine, Oxidative Stress, protein oxidation

Anti-7-Ketocholesterol (7-KC) monoclonal antibody

mars 10 | Oxidative Stress, Products | admin

About 7-ketocholesterol

Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.

7-Ketocholesterol

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02-1301: Anti 7-ketocholesterol (7-KC) monoclonal antibody (20µg)

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Tagged 7-ketocholesterol, lipid peroxydation, Oxidative Stress

Anti-Methyglyoxal (MG) monoclonal antibody

mars 10 | Oxidative Stress, Products | admin

About Methyglyoxal (MG)

Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.

Methylglyoxal Adduct

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02-1201: Anti-methylglyoxal monoclonal antibody

Tagged lipid peroxydation, methylglyoxal, Oxidative Stress

Anti-Crotonaldehyde (CRA) monoclonal antibody

mars 10 | Oxidative Stress, Products | admin

About crotonaldehyde (CRA)

Crotonaldehyde (CRA) is a representative carcinogenic aldehyde formed endogenously through lipid peroxidation. CRA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with CRA and lysine residue leads to the formation of numerous numbers of adducts. This antibody is specific for the CRA-modified protein.

Crotonaldehyde

Product Specification

02-1101: Anti-Crotonaldehyde (CRA) monoclonal antibody (30µg)

Tagged CRA, crotonaldehyde, lipid peroxydation, Oxidative Stress

Anti-4-hydroxy-2-hexenal (4-HHE) monoclonal antibody

mars 10 | Oxidative Stress, Products | admin

About 4-hydroxy-2-hexenal (4-HHE)

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.

4-hydroxy-2-hexenal adduct

Product Information

02-1001: Anti-4-hydroxy-2-hexenal (4-HHE) monoclonal antibody (30µg)

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Tagged 4-HHE, 4-hydroxy-2-hexenal, lipid peroxydation, Micheal Adduct, Oxidative Stress

Products

Hexanoyl-lysine: A novel biomarker for early stage lipid peroxydation

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Hexanoyl-Lysine Adduct: A early stage of lipid oxidation

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About 7-ketocholesterol

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About Methyglyoxal (MG)

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About crotonaldehyde (CRA)

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About 4-hydroxy-2-hexenal (4-HHE)

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