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Oxidative Stress

Oxidative Stress Products

octobre 17  |  news, Oxidative Stress, Products  |   admin

Oxidative stress represents an imbalance between reactive oxygen species (ROS) and cell system’s ability to detoxify the reactive intermediates or to repair the resulting damage. Disturbances in the normal state can cause toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids, and DNA.

Nowadays, oxidative stress may involved in cell perturbation leading to various diseases like atherosclerosis, heart failure, myocardial infarction, Alzheimer’s disease, schizophrenia…

Advanced BioDesign offer you a wide range of product in order to monitor oxidative stress in your cells or tissues following specific marker:

Download our product flyer: Oxidative Stress Product.

Advanced BioDesign Team

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Hexanoyl-lysine (HEL) monoclonal antibody

mars 23  |  Oxidative Stress, Products  |   admin

Hexanoyl-lysine: A novel biomarker for early stage lipid peroxydation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HpODE) is found to be covalently bound to proteins. Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

 

Product Information

  • 02-0702: Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) (20µg)

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Hexanoyl-Lysine Adduct(HEL) ELISA kit

mars 14  |  Oxidative Stress, Products  |   admin

Hexanoyl-Lysine Adduct: A early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

Hexanoyllsyine formation process

Product Information

  • 02-0701: Hexanoyl-Lys(HEL) ELISA kit

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Anti-7-Ketocholesterol (7-KC) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About 7-ketocholesterol

Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.

7-Ketocholesterol

Product Information

  • 02-1301: Anti 7-ketocholesterol (7-KC) monoclonal antibody (20µg)

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Anti-Methyglyoxal (MG) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About Methyglyoxal (MG)

Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common intermediate in the Maillard reaction (glycation), reacts with proteins and forms advanced glycation end products (AGE). MG reacts with arginine residue in protein and forms numerous numbers of adducts, such as argpyrimidine. This antibody is specific for argpyrimidine: N-(5-hydroxy-4,6-dimethylpyrimidine-2-yl)-L-ornithine.

Methylglyoxal Adduct

Product Information

  • 02-1201: Anti-methylglyoxal monoclonal antibody

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Anti-Crotonaldehyde (CRA) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About crotonaldehyde (CRA)

Crotonaldehyde (CRA) is a representative carcinogenic aldehyde formed endogenously through lipid peroxidation. CRA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with CRA and lysine residue leads to the formation of numerous numbers of adducts. This antibody is specific for the CRA-modified protein.

Crotonaldehyde

Product Specification

  • 02-1101: Anti-Crotonaldehyde (CRA) monoclonal antibody (30µg)

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Anti-4-hydroxy-2-hexenal (4-HHE) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About 4-hydroxy-2-hexenal (4-HHE)

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.

4-hydroxy-2-hexenal adduct

Product Information

02-1001: Anti-4-hydroxy-2-hexenal (4-HHE) monoclonal antibody (30µg)

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Anti-Acrolein (ACR) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About Acrolein (ACR)

Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Acrolein Adduct

Product Information

  • 02-0901: Anti-Acrolein (ACR) monoclonal antibody (20µg)

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Anti-malondialdehyde (MDA) monoclonal antibody

mars 10  |  Oxidative Stress, Products  |   admin

About malondialdehyde (MDA)

Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

Malondialdehyde adduct

Product Information

  • 02-0801: Anti-Malonaldehyde antibody (30µg)

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8-OHdG ELISA Kit

février 24  |  Oxidative Stress, Products  |   admin

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.

Formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) by oxygen radicals. H Kasai Environmental Mutagen Research, Vol 10, p73-78(1988)

This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use ‘Highly Sensitive 8-OHdG Check ELISA.

  1. High specificity & high sensitivity: Cross reactivity was checked for 19 analogues.
  2. Easy operation & speedy: There is no need for expensive equipment and sample pretreatment. Required equipments are: pipettes, incubator and micro plate reader with 450nm filter. Assays can be completed within 3.5 hours (New 8­OHdG Check).

Product Information

  • 02-0101: 8-OHdG Check ELISA kit
  • 02-0102: Highly Sensitive 8-OHdG Check ELISA kit
  • 02-0103: 8-OHdG Check ELISA Trial Package (32wells)

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