Advanced BioDesign HISbind Plate Kit provides a platform for capture, enzymatic assay and purification of recombinant histidine tagged protein.
Protein Research
EIA HRP Substrate Kit
Horseradish Peroxydase (HRP) is a 40kDa protein that catalyzes the oxidation of hydrogen peroxide (H2O2) that, in the presence of substrate, results in a colored or fluorescent product. HRP is one of the most commonly used protein-markers in protein research and TMB (3, 3’, 4, 4’ – tetramethylbenzidine) is the most common substrate used in ELISA. Reaction between the acceptor and oxidized H2O2 will produce a blue colored solution. Once the desired intensity is obtained, the reaction is stopped with an acidic solution (STOP Solution). The reaction’s intensity can be monitored with a 96 well plate reader at 450nm.
Tagged EIA, ELISA, HRP Substrate, TMB
ECL Substrate Kit
Advanced BioDesign ECL Western Blotting Substrate is using a new luminol enhancer for boosting signals without increasing the background.
Tagged chemiluminescent, ECL, HRP Substrate, Western Blot
AminoBind Plate Kit
Product Reference
- 01-0101 – AminoBind Plate Kit
Advanced BioDesign AminoBind Plate allow the immobilization of primary amine containing molecule, like peptide with free N-terminus… Primary amine will react with succinmidyl ester to form an amide bound (see figure).
This plate type will be convenient for peptide or molecule which do not efficiently coat by passive adsorption.
Tagged Amines, AminoBind, Protein Research
SulfoBind Plate Kit
Product Reference
- 01-0101 – SulfoBind Plate Kit
SulfoBind Plate is maleimide activated plate. This linker makes able the covalently coupling of sulfhydryl containing molecule, such as Cysteine in peptide, to plate.Maleimide group will form a stable thioether bond with sulfhydryl group (see figure). This linkage is easy and rapid to form.
Tagged Cysteine, Protein Research, Sulfhydryl
