Advanced BioDesign’s presence at B4B days is confirmation of its ambition to become one of the major providers of novel, specialized reagents for oncology research. During these two days, we will be at your disposal to discuss your research projects with the aim of providing you with the tools that are the most appropriate for your research. We will share with you our expertise in the development of monoclonal antibodies including that of customized kits. We look forward to welcoming you to our booth for face-to-face meetings.

Link to the event.

Highlights

• Easy binding of biotin-containing conjugates,
• Low nonspecific binding,
• Ideal for small biotinylated molecules (e.g. peptides, DNA, RNA…),
• Ideal for protein immobilizations without denaturing.

Product Detail

• Binding Capacity: 200 pmol/well (biotin),
• Binding Sensibility: 5 pmol/well (biotin),
• Coating Volume: 100µL,
• Blocking Volume: 300µL.

Product Reference

7th Cancer Scientific Forum of the Cancéropôle CLARA is one of the main French’s event in Oncology research. To be a significant actor in this domain, we will be present.

We invite you to contact us to discuss about new potential projects. Please send us an email at contact@a-biodesign.com.

This accreditation delivered by the french research ministry, spolight our competitivity to develop customer R&D project, particulary in Immunotechnology.

This measures allow to French Company a deduction of costs, thus develop new industrial collaborations.

Nowadays, oxidative stress may involved in cell perturbation leading to various diseases like atherosclerosis, heart failure, myocardial infarction, Alzheimer’s disease, schizophrenia…

The six consecutive histidine residues take place on the N or C terminus of protein. This amino acid will form an electronegative cloud that can be bind by positively charged metal ions such as nickel. This linkage can be broken using high concentration of imidazole result the release of the tagged protein.

This product may be used for monitor His-tagged protein such as expression screening, immunoassays, competition assays, biochemical assays or protein purification.

The limit of detection of this product is 10pg of His-tagged protein per well.

Highlights

• Detection limit: 10pg of histidine tagged protein.
• Detergents do not interact with the binding capacity of the plate.
• Pre-blocked plate for reducement of non-specific binding
• Use optimizes Wash Buffer for lower background.
• Can be used for little purification of histidine-tagged protein.
• This plate can be chelated with other metals ions. Please contact our technical services for any request.

Product Reference

Highlights

• Ready to use HRP Substrate
• Sensitivity (ng)

Product Reference

Using this new substrate is also a reliable low cost solution for enhanced chemiluminescence (ECL) that will replace more expensive products without losing sensibility.

ABD uses in this substrate a new luminol enhancer for boosting signals without increasing the background.

Highlights

• Low cost ECL Substrate: cost less than similar chemiluminescent substrates.
• New Luminol Enhancer: switching to ABD ECL substrate for Western blot detections yielding strong signals and low background.
• Saving antibody: requires less primary and/or secondary antibodies per blot.

Product Reference

Advanced BioDesign Team

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HpODE) is found to be covalently bound to proteins. Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

Product Information

• 02-0702: Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) (20µg)

Product Specification

• Clone: 5F12
• Immunogen: N-ε-Hexanoyl modified keyhole limpet hemocyanin (KLH)
• Specificity: Specific to hexanoyl adduct. Cross reactivity has been checked for following oxidized lipids: MDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonnanal, 2-nonenal & HNE
• Subclass: Mouse IgG1(κ)
• Form: Lyophilized powder, containing 100mM PBS, 5% sucrose, 1% BSA
• Applications: Immunohistochemistry (recommended concentration 2µg/mL)
• Storage: Store at less than  -20°C

Detection of HEL at Human atherosclerotic lesions.

References

• Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
• Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
• Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
• Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
• Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)