protein oxidation

Hexanoyl-lysine (HEL) monoclonal antibody

mars 23  |  Oxidative Stress, Products  |   admin

Hexanoyl-lysine: A novel biomarker for early stage lipid peroxydation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HpODE) is found to be covalently bound to proteins. Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

 

Product Information

  • 02-0702: Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) (20µg)

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Hexanoyl-Lysine Adduct(HEL) ELISA kit

mars 14  |  Oxidative Stress, Products  |   admin

Hexanoyl-Lysine Adduct: A early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

Hexanoyllsyine formation process

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  • 02-0701: Hexanoyl-Lys(HEL) ELISA kit

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Anti-Dityrosine (DT) monoclonal antibody

février 24  |  Oxidative Stress, Products  |   admin

About Dityrosine: A novel biomarker for protein oxidation

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine (DT) and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. Dityrosine have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.

Dityrosine A novel biomarker for protein oxydation

Product Information

  • 02-0601: Anti-DiTyrosine (DT) monoclonal antibody

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